منابع مشابه
Cellulose acetate as solid phase in ELISA for plague.
Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 microg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixivia...
متن کاملEvaluation of a standardized F1 capsular antigen capture ELISA test kit for the rapid diagnosis of plague.
Rapid detection of soluble F1 capsular antigen in serum, bubo fluid or urine of patients proved to be a valuable tool in the presumptive diagnosis of plague. We evaluated a F1 capsular antigen capture ELISA resembling a commercially available test kit. The minimal detectable concentration was 4 ng/ml. The specificity was 100% when investigating 47 sera from healthy Malagasy subjects and 98.4% w...
متن کاملRapid ELISA for the diagnosis of rotavirus.
BACKGROUND & OBJECTIVES Rotavirus is the major cause of gastroenteritis in infants and young children all over the world. The objective of the study was to develop a rapid ELISA for the diagnosis of rotavirus infection in children hospitalised with diarrhoea. METHODS Immune serum was raised in rabbits by inoculating semipurified rotavirus, SA-11 strain. Immunoglobulins were conjugated to hors...
متن کاملDevelopment of a Nano-ELISA system for the rapid and sensitive detection of H9N2 avian influenza
Influenza is one of the most important viral diseases that is common among the birds and the mammalsand is caused by specific viruses that belong to the Orthomyxoviridae family. Migratory aquatic birds arethe reservoir of the disease and there is a likelihood of the disease in any region. There are differentmethods for detecting the avian influenza, but by the point of detection rates, the ELIS...
متن کاملa rapid and specific pcr–elisa for detecting salmonella typhi
background salmonella continues to be a major food borne pathogen for animals and humans. a sensitive and specific pcr–elisa technique was developed to detect salmonella typhi. materials and methods the assay was based on the incorporation of digoxigenin-labeled dutp and a biotin-labeled primers specific for rfbe gene during pcr amplification. the labeled pcr products were bound to streptoavidi...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 1998
ISSN: 0074-0276
DOI: 10.1590/s0074-02761998000100020